| 质粒名称: | pCMV-MCP-HA-EEEV nsP3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002870 |
| 产品用途: | Mammalian expression of MCP-HA-EEEV nsP3 |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EEEV nsP3 |
| 物种来源: | Alphavirus |
| 密码子优化: | 大肠杆菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Alphaviral non-structural protein-host RBP co-condensation as a mechanism to sustain virus replication
Molecular Cell, Volume 86, Issue 9, 2026
Yi Liu, Peiguo Yang
|
pCMV-MCP-HA-EEEV nsP3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCMV-MCP-HA-EEEV nsP3载体质粒基本信息
pCMV-MCP-HA-EEEV nsP3质粒图谱载体图谱和pCMV-MCP-HA-EEEV nsP3载体序列质粒序列多克隆位点信息
pCMV-MCP-HA-EEEV nsP3质粒载体简介
pCMV-MCP-HA-EEEV nsP3质粒序列载体序列
LOCUS pCMV-MCP-HA-EEEV nsP3 6042 bp DNA circular SYN 16-JUL-2026
DEFINITION Localization: C1 Cloning Vector, Excitation: 488, Emission: 507.
ACCESSION .
VERSION .
KEYWORDS pCMV-MCP-HA-EEEV nsP3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6042)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 6042)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 7, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6042
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 142..445
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 446..649
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 600..620
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind complement(672..708)
/label=引物 6
primer_bind 679..717
/label=引物 1
regulatory 688..693
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 694..1080
/codon_start=1
/label=MCP
/translation="MLAVKMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRS
QAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGL
LKDGNPIPSAIAANSGIYADPPVAT"
CDS 1093..1119
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(1117..1140)
/label=p61-1060
primer_bind 1126..1163
/label=p61-1053
CDS 1141..2799
/codon_start=1
/label=EEEV nsP3
/translation="APAYRVVRGDITKSNDEVIVNAANNKGQPGSGVCGALYRKWPGAF
DKQPVATGKAHLVKHSPNVIHAVGPNFSRLSENEGDQKLSEVYMDIARIINNERFTKVS
IPLLSTGIYAGGKDRVMQSLNHLFTAMDTTDADITIYCLDKQWESRIKEAITRKESVEE
LTEDDRPVDIELVRVHPLSSLAGRPGYSTTEGKVYSYLEGTRFHQTAKDIAEIYAMWPN
KQEANEQICLYVLGESMNSIRSKCPVEESEASSPPHTIPCLCNYAMTAERVYRLRMAKN
EQFAVCSSFQLPKYRITGVQKIQCSKPVIFSGTVPPAIHPRKFASVTVEDTPVVQPERL
VPRRPAPPVPVPARIPSPPCTSTNGSTTSIQSLGEDQSASASSGAEISVDQVSLWSIPS
ATGFDVRTSSSLSLEQPTFPTMVVEAEIHASQGSLWSIPSITGSETRAPSPPSQDSRPS
TPSASGSHTSVDLITFDSVAEILEDFSRSPFQFLSEIKPIPAPRTRVNNMSRSADTIKP
IPKPRKCQVKYTQPPGVARVISAAEFDEFVRRHSN"
misc_feature 2551..2589
/label=G3BP binding motif
misc_feature 2731..2781
/label=FXR binding motif
primer_bind complement(2777..2814)
/label=p61-1054
primer_bind 2800..2825
/label=p61-1059
polyA_signal 2911..3032
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2948..2967)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3002..3021
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(3039..3494)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3176..3197)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3388..3407
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3521..3625
/gene="bla"
/label=AmpR promoter
promoter 3627..3984
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3650..3670)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3835..3970
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3897..3916
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3920..3931)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4019..4813
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4073..4092)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4683..4702
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5001..5020)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5045..5092
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5421..6009
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5910..5929
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
61 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
121 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
181 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
241 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
301 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
361 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
421 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
481 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
541 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
601 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
661 ccgtcagatc cgctagcgct accggtcgcc accatgctag ccgttaaaat ggcttctaac
721 tttactcagt tcgttctcgt cgacaatggc ggaactggcg acgtgactgt cgccccaagc
781 aacttcgcta acgggatcgc tgaatggatc agctctaact cgcgttcaca ggcttacaaa
841 gtaacctgta gcgttcgtca gagctctgcg cagaatcgca aatacaccat caaagtcgag
901 gtgcctaaag gcgcctggcg ttcgtactta aatatggaac taaccattcc aattttcgcc
961 acgaattccg actgcgagct tattgttaag gcaatgcaag gtctcctaaa agatggaaac
1021 ccgattccct cagcaatcgc agcaaactcc ggcatctacg cggatccacc ggtcgccacc
1081 ccggtcgcca cctatccata tgacgttcca gattacgctt actcagatct cgagctcaag
1141 gcacctgcat atcgcgtagt gcgaggtgac attaccaaga gcaacgatga ggtcattgtg
1201 aacgcagcaa acaataaagg acaacctggc tctggggtgt gcggtgctct gtatcggaaa
1261 tggccagggg cttttgataa acaacctgtc gccaccggta aggctcatct ggtgaaacac
1321 agccctaacg tcattcacgc cgtagggccc aattttagtc ggctgtcaga aaatgaaggg
1381 gatcaaaagc tctccgaagt gtacatggac atagcaagaa taattaataa tgagcggttc
1441 accaaagtca gcattcctct gctgtccact ggcatatatg ctggggggaa ggacagggtt
1501 atgcaaagtc tgaaccacct cttcaccgca atggacacga cagatgccga catcactatt
1561 tactgtttgg ataaacaatg ggagtctagg attaaggaag ccatcacacg caaggagtct
1621 gtggaggaac tgaccgagga tgataggcca gtagacattg aactggtgcg cgtgcacccc
1681 ctctcttcac ttgccggtag acccgggtat agtactaccg agggtaaggt ctacagctac
1741 ctggagggta cccgcttcca tcaaaccgcc aaggatatcg ccgaaattta tgcaatgtgg
1801 ccaaataagc aggaggccaa cgaacagatc tgtctttacg tactgggcga gtcaatgaat
1861 tccatccgca gcaaatgccc agtcgaagaa tctgaagcct cctcccctcc tcatacaata
1921 ccctgtctgt gcaattacgc catgaccgct gagcgcgttt atcggctgcg catggccaag
1981 aacgagcagt ttgccgtatg tagcagtttc cagctgccaa agtatcgaat cacaggcgtc
2041 caaaaaattc aatgttcaaa gcctgttatt ttctctggaa ctgtcccgcc ggcaatccat
2101 cccagaaagt ttgcttcagt cacagttgag gatactcctg ttgtgcagcc agagaggctc
2161 gtgcctcgca gaccagctcc cccagttcca gttcccgctc gaataccttc acccccatgc
2221 acctccacca acggttccac gacctccatc cagtccctcg gcgaggatca gagcgccagt
2281 gctagttccg gggctgaaat cagcgtggac caggtgagcc tgtggtctat accctctgct
2341 actggattcg acgtaagaac tagcagctca ctttccctcg aacagcccac tttcccaaca
2401 atggtcgtgg aggccgaaat tcacgccagc cagggctctc tgtggtccat tcctagcatt
2461 acaggatctg aaacgagggc acccagccca ccctcacagg atagtaggcc atcaacaccc
2521 tctgcaagcg gttcccatac atccgtagat ttgattacct tcgactccgt agccgagatt
2581 ctggaggact ttagcaggtc tccctttcaa ttcctgtccg agatcaagcc catacccgcc
2641 ccaaggacac gcgtgaataa tatgtcacgc agcgccgata caattaaacc aattcccaag
2701 ccacgcaagt gtcaggttaa atatacccaa ccgcctggtg ttgctcgggt catcagtgcg
2761 gccgaattcg acgagtttgt gcgccggcac tccaattgat aactgatcat aatcagccat
2821 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
2881 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
2941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
3001 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
3061 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
3121 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
3181 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
3241 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
3301 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
3361 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
3421 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
3481 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
3541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
3601 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
3661 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
3721 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
3781 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
3841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
3901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
3961 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
4021 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
4081 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
4141 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
4201 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
4261 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
4321 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
4381 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
4441 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
4501 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
4561 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
4621 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
4681 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
4741 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
4801 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
4861 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
4921 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
4981 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
5041 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
5101 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
5161 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
5221 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
5281 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
5341 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
5401 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
5461 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
5521 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
5581 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
5641 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
5701 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
5761 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
5821 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
5881 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
5941 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
6001 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tg
//
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